|
|
|
Friday, Mar.29, 2024
|
|
|
|
|
|
miRNA: |
hsa-miR-223 |
Disease: |
hepatocellular carcinoma (HCC) |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
Expression pattern of miRNA: |
down-regulated |
Validated targets of miRNA from the reference: |
STMN1 |
Validated targets of miRNA from TarBase: |
NFIA : More... |
Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: The highly deregulated miR-223 and miR-222 could unequivocally distinguish HCC from adjacent nontumoral liver, irrespective of viral associations (P=.0002). Re-expression of miR-223 in HBV, HCV, and non-HBV non-HCV-related HCC cell lines revealed a consistent inhibitory effect on cell viability (P<.01). Integrative analysis further implicated Stathmin 1 (STMN1) as a downstream target of miR-223. A strong inverse relationship between STMN1 mRNA and miR-223 expressions was shown (P =.006). At the protein level, restoration of miR-223 expressions in HCC cell lines led to a substantial reduction in STMN1 protein expression. We further showed that miR-223 readily could suppress the luciferase activity in reporter construct containing the STMN1 3' untranslated region (P =.02). |
Reference:
MicroRNA-223 is commonly repressed in hepatocellular carcinoma and potentiates expression of Stathmin1. | PMID:18555017
Wong QW, Lung RW, Law PT, Lai PB, Chan KY, To KF, Wong N.
Gastroenterology. 2008 Jul;135(1):257-69. Epub 2008 Apr 11. |
|
|
|
|
|
|