hsa-miR-29c

nasopharyngeal carcinoma (NPC)

Relationship type:

Causal

Detection method for miRNA expression:

Northern blot, qRT-PCR etc

down-regulated

Validated targets of miRNA from the reference:

COL1A1, COL1A2, COL3A1, COL4A1, COL4A2, COL15A1, FUSIP1iso1, LAMININ gamma1, SPARC, TDG

Validated targets of miRNA from TarBase:

unknown : More...

Description:

In NPC tumors, the lower mir-29c levels correlated with higher levels of multiple mRNAs whose 3 UTRs can bind mir-29c at target sequences conserved across many vertebrates. In cultured cells, introduction of mir-29c down-regulated these genes at the level of mRNA and inhibited expression of luciferase encoded by vectors having the 3 UTRs of these genes. Moreover, for each of several genes tested, mutating the mir-29c target sites in the 3 UTR abrogated mir-29c induced inhibition of luciferase expression. Most of the mir-29ctargeted genes identified encode extracellular matrix proteins, including multiple collagens and laminin 1, that are associated with tumor cell invasiveness and metastatic potential, prominent characteristics of NPC. The 3 UTRs of all of these 10 candidate target genes (Collagen 1A1, 1A2, 3A1, 4A1, 4A2, 15A1, FUSIP1iso1, laminin 1, SPARC, and TDG) elicited significantly decreased luciferase activities in mir-29c transfected cells.

Reference:

MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins. | PMID:18390668
Sengupta S, den Boon JA, Chen IH, Newton MA, Stanhope SA, Cheng YJ, Chen CJ, Hildesheim A, Sugden B, Ahlquist P.
Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5874-8. Epub 2008 Apr 4.