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Friday, Apr.26, 2024
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| miRNA: |
hsa-miR-34a |
| Disease: |
neuroblastoma (NB) |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
| Expression pattern of miRNA: |
down-regulated |
Validated targets of miRNA from the reference: |
MYCN |
Validated targets of miRNA from TarBase: |
E2F3 : More... |
| Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: miR-34a caused the most significant suppression of cell growth through increased apoptosis and decreased DNA synthesis in neuroblastoma cell lines with MYCN amplification. Quantitative RT-PCR showed that neuroblastoma tumors with 1p36 loss expressed lower level of miR-34a than those with normal copies of 1p36. Furthermore, we demonstrated that MYCN is a direct target of miR-34a. Finally, using a series of mRNA expression profiling experiments, we identified other potential direct targets of miR-34a, and pathway analysis demonstrated that miR-34a suppresses cell-cycle genes and induces several neural-related genes. This study demonstrates one important regulatory role of miR-34a in cell growth and MYCN suppression in neuroblastoma. |
Reference:
The MYCN oncogene is a direct target of miR-34a. | PMID:18504438
Wei JS, Song YK, Durinck S, Chen QR, Cheuk AT, Tsang P, Zhang Q, Thiele CJ, Slack A, Shohet J, Khan J.
Oncogene. 2008 May 26. [Epub ahead of print] |
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