Description: Real-time reverse transcriptase polymerase chain reaction (RT-PCR) validation of microarray. Realtime
semiquantitative RT-PCR of five different microRNA species (miR-21, miR-34a, miR-125b, miR-139, and miR-323) across ten subjects. miR-21, miR-34a, miR-125b, and miR-323 were overexpressed in leiomyoma in the ten pairs tested and miR-139 was underexpressed, consistent with the microarray results. |