hsa-let-7b

malignant melanoma

Relationship type:

Causal

Detection method for miRNA expression:

Northern blot, qRT-PCR etc

down-regulated

Validated targets of miRNA from the reference:

CCND1

Validated targets of miRNA from TarBase:

Lin28,Mtpn : More...

Description:

Immunoblot and immunofluorescence analyses showed that overexpression of let-7b in melanoma cells resulted in significant downregulation of cyclins D1, D3, and Cdk4 protein expression. Downregulation of cyclin A protein expression was less prominent. Moreover, the negative regulatory effect of let-7b on cyclin D1 expression was indeed mediated through binding to 3′UTR of the target, as shown by in vitro luciferase assays.let-7b might be an important negative regulator of melanoma cell growth and proliferation via inhibition of cell cycle progression.

Reference:

MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth. | PMID:18379589
Schultz J, Lorenz P, Gross G, Ibrahim S, Kunz M.
Cell Res. 2008 May;18(5):549-57.