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Friday, May.3, 2024
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miRNA: |
hsa-miR-222 |
Disease: |
non-small cell lung cancer (NSCLC) |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
Expression pattern of miRNA: |
up-regulated |
Validated targets of miRNA from the reference: |
Kit, p27(kip1) |
Validated targets of miRNA from TarBase: |
KIT : More... |
Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: We demonstrate that these miR-221 and miR-222 impair TRAIL-dependent apoptosis by inhibiting the expression of key functional proteins. Indeed, transfection with anti-miR-221 and -222 rendered CALU-1-resistant cells sensitive to TRAIL. Conversely, H460-sensitive cells treated with -221 and -222 pre-miRs become resistant to TRAIL. miR-221 and -222 target the 3'-UTR of Kit and p27(kip1) mRNAs, but interfere with TRAIL signaling mainly through p27(kip1). In conclusion, we show that high expression levels of miR-221 and -222 are needed to maintain the TRAIL-resistant phenotype, thus making these miRs as promising therapeutic targets or diagnostic tool for TRAIL resistance in NSCLC. |
Reference:
MicroRNA signatures of TRAIL resistance in human non-small cell lung cancer. | PMID:18246122
Garofalo M, Quintavalle C, Di Leva G, Zanca C, Romano G, Taccioli C, Liu CG, Croce CM, Condorelli G.
Oncogene. 2008 Jun 19;27(27):3845-55. Epub 2008 Feb 4. |
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