| miRNA: |
hsa-let-7a |
| Disease: |
malignant melanoma |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
| Expression pattern of miRNA: |
down-regulated |
Validated targets of miRNA from the reference: |
integrin beta(3) |
Validated targets of miRNA from TarBase: |
NF2 : More... |
| Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: Transfection of melanoma cells with let-7a pre-miR(TM) molecules resulted in the downregulation of integrin beta(3) mRNA and protein expression. In addition, we cloned the 3'-UTR of the integrin beta(3) mRNA containing the let-7a target sequence into a reporter plasmid and revealed that let-7a negatively regulates reporter gene expression. The repressed expression of integrin beta(3) accompanies with reduced invasive potential of melanoma cells transfected with synthetic let-7a molecules observed in Boyden chamber assays. On the other hand, the induction of integrin beta(3) expression was achieved in melanocytes by transfection with let-7a anti-miRs, resulting in invasive behavior of transfected melanocytes. In summary, we determined miRNA let-7a to be an important regulator of integrin beta(3) expression and showed that the loss of let-7a expression is involved in development and progression of malignant melanoma. |
Reference:
Integrin beta(3) expression is regulated by let-7a miRNA in malignant melanoma. | PMID:18679415
Müller DW, Bosserhoff AK.
Oncogene. 2008 Aug 4. [Epub ahead of print] |
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