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Monday, May.20, 2024
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| miRNA: |
hsa-miR-196a |
| Disease: |
esophageal cancer |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
| Expression pattern of miRNA: |
up-regulated |
Validated targets of miRNA from the reference: |
ANXA1 |
Validated targets of miRNA from TarBase: |
unknown : More... |
| Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: Analysis of paired normal/tumor tissues from additional 10 patients revealed an increase in miR-196a in the cancers (P=0.003), accompanied by a decrease in ANXA1 mRNA (P=0.004). Increasing miR-196a levels in cells by miR-196a mimics resulted in decreased ANXA1 mRNA and protein. In addition, miR-196a mimics inhibited luciferase expression in luciferase plasmid reporter that included predicted miR-196a recognition sequence from ANXA1 3'-untranslated region confirming that miR-196a directly targets ANXA1. miR-196a promoted cell proliferation, anchorage-independent growth and suppressed apoptosis, suggesting its oncogenic potential. This study demonstrated a novel mechanism of post-transcriptional regulation of ANXA1 expression and identified miR-196a as a marker of esophageal cancer. |
Reference:
MicroRNA-196a targets annexin A1: a microRNA-mediated mechanism of annexin A1 downregulation in cancers. | PMID:18663355
Luthra R, Singh RR, Luthra MG, Li YX, Hannah C, Romans AM, Barkoh BA, Chen SS, Ensor J, Maru DM, Broaddus RR, Rashid A, Albarracin CT.
Oncogene. 2008 Jul 28. [Epub ahead of print] |
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