hsa-miR-19a

multiple myeloma (MM)

Relationship type:

Causal

Detection method for miRNA expression:

Northern blot, qRT-PCR etc

up-regulated

Validated targets of miRNA from the reference:

SOCS-1, BIM

Validated targets of miRNA from TarBase:

PTEN : More...

Description:

Our findings indicate that miR-19a and b are up-regulated >100-fold in patient samples, >2,000 times in the cell lines , and are both almost absent in normal PCs and MGUS. These data suggest that both miRNAs contribute to the development of MM. miR-19a and miR-19b Target SOCS-1, a Negative Regulator of IL-6R/STAT-3 Pathway. Moreover, miR-19a and miR-19b mimics inhibited the expression of a reporter vector containing SOCS-1 3 UTR, while mutation of the predicted miRNA-binding site abrogated this effect. These studies suggest a role of miR-19s in the IL-6 antiapoptotic signal in the pathogenesis and malignant growth of MM. U266 cells were transfected with miR-19s ASOs and BIM expression was evaluated using immunoblot. We found a significant increase of BIM protein levels at 48 h after treatment with anti-miR-19s compared to scrambled oligonucleotides.BIM as a direct target of miR-17-92 and suggest a possible mechanism through which over-expression of miR-17 92 contributes to the antiapoptotic signals in MM.

Reference:

MicroRNAs regulate critical genes associated with multiple myeloma pathogenesis. | PMID:18728182
Pichiorri F, Suh SS, Ladetto M, Kuehl M, Palumbo T, Drandi D, Taccioli C, Zanesi N, Alder H, Hagan JP,
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12885-90. Epub 2008 Aug 26.