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Thursday, Mar.28, 2024
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miRNA: |
hsa-miR-20a |
Disease: |
B-cell lymphoma |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
Expression pattern of miRNA: |
up-regulated |
Validated targets of miRNA from the reference: |
CDKN1A, p21 |
Validated targets of miRNA from TarBase: |
TGFBR2 : More... |
Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: To identify essential target(s) of miR-17-92 in lymphomagenesis, we first transfected miR-17-92 into two genetically distinct B-cell lymphoma cell lines: Raji cells, which overexpress c-Myc, and SUDHL4 cells, which overexpress Bcl2. Raji cells transfected with miR-17-19b-1 exhibited downregulated expression Bim and a slight upregulation in Bcl2 expression. On the other hand, SUDHL4 cell transfectants showed aggressive cell growth reflecting facilitated cell cycle progression at the G1-S transition and decreased expression of CDKN1A mRNA and p21 protein (CDKN1A/p21) that was independent of p53 expression. Conversely, transfection of antisense oligonucleotides against miR-17 and miR-20a into Jeko-1 cells led to upregulation of CDKN1A/p21, resulting in decreased cell growth with G1-S arrest. Thus, CDKN1A/p21 appears to be an essential target of miR-17-92 during B-cell lymphomagenesis, which suggests the miR-17-92 polycistron has distinct targets in different B-cell lymphoma subtypes. |
Reference:
MicroRNA-17-92 downregulates expression of distinct targets in different B-cell lymphoma subtypes. | PMID:18941111
Inomata M, Tagawa H, Guo YM, Kameoka Y, Takahashi N, Sawada K.
Blood. 2008 Oct 21. [Epub ahead of print] |
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