Friday, Mar.29, 2024
miRNA:

hsa-miR-155

Disease:

Waldenstrom Macroglobulinemia (WM)

Relationship type:

Unspecified

Detection method for miRNA expression:

Northern blot, qRT-PCR etc

Expression pattern of miRNA:

up-regulated

Validated targets of miRNA from the reference:

unknown

Validated targets of miRNA from TarBase:

AGTR1 : More...

Predicted targets: MIRANDA, TARGETSCAN, PICTAR-VERT

 

Description:

Multi-level genetic characterization of Waldenstrom Macroglobulinemia (WM) is required to improve our understanding of the underlying molecular changes that lead to the initiation and progression of this disease. We performed microRNA-expression-profiling of bone marrow-derived CD19(+) WM cells, compared to their normal cellular counterparts and validated data by qRT-PCR. We identified a WM-specific microRNA signature characterized by increased expression of microRNA-363*/-206/-494/-155/-184/-542-3p; and decreased expression of microRNA-9* (ANOVA; P<0.01). We found that microRNA-155 regulates proliferation and growth of WM cells in vitro and in vivo, by inhibiting MAPK/ERK, PI3/AKT and NF-kB pathways. Potential microRNA-155 target genes were identified using gene-expression-profiling and included genes involved in cell cycle progression, adhesion, and migration. Importantly, increased expression of the 6 miRNAs significantly correlated with a poorer outcome predicted by the International-Prognostic-Staging-System for WM. We further demonstrated that therapeutic agents commonly used in WM (rituximab/perifosine/bortezomib) alter the levels of the major miRNAs identified, by inducing down-modulation of five increased miRNAs (all but miR-206) and up-modulation of patient-downexpressed miRNA-9*. These data indicate that microRNAs play a pivotal role in the biology of WM; represent important prognostic marker; and provide the basis for the development of new microRNA-based targeted therapies in WM.

 

 

Reference:

microRNA expression in the biology, prognosis and therapy of Waldenstrom macroglobulinemia. | PMID:19074725
Roccaro AM, Sacco A, Chen C, Runnels J, Leleu X, Azab F, Azab AK, Jia X, Ngo HT, Melhem MR, Burwick N, Varticovski L, Novina CD, Rollins BJ, Anderson KC, Ghobrial IM.
Blood. 2008 Dec 22. [Epub ahead of print]

 

 
 
 
  miRBase
  Tarbase
  miRGen
  miRGator
  CCBB
  MicroRNA.org
  miRRim
  miRNAMap 2.0
 
 
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