| miRNA: |
hsa-miR-27a |
| Disease: |
gastric cancer (stomach cancer) |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
| Expression pattern of miRNA: |
up-regulated |
Validated targets of miRNA from the reference: |
prohibitin |
Validated targets of miRNA from TarBase: |
unknown : More... |
| Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: miR-27a is up-regulated in human gastric adenocarcinoma. Suppression of miR-27a inhibits gastric cancer cell growth. Subsequently, prohibitin is identified as a potential miR-27a target, combining bioinformatics and microarray analysis. EGFP report experiment also confirms that the 3' untranslated region (3'UTR) of prohibitin carries the directly binding site of miR-27a. After knockdown of miR-27a in gastric cancer cells, mRNA level and protein level of prohibitin are both elevated. Down-regulation of prohibitin by miR-27a may explain why suppression of miR-27a can inhibit gastric cancer cell growth, further supporting that miR-27a functions as an oncogene. |
Reference:
MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin. | PMID:18789835
Liu T, Tang H, Lang Y, Liu M, Li X.
Cancer Lett. 2009 Jan 18;273(2):233-42. Epub 2008 Sep 13. |
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