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Thursday, Apr.25, 2024
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| miRNA: |
hsa-miR-34a |
| Disease: |
melanoma |
Relationship type: |
Causal |
Detection method for miRNA expression: |
northern blot, qRT-PCR etc |
| Expression pattern of miRNA: |
down-regulated |
Validated targets of miRNA from the reference: |
c-Met |
Validated targets of miRNA from TarBase: |
E2F3 : More... |
| Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: miR-34a is actively expressed in melanocytes but not in uveal melanoma cells based on Northern blot analysis. Transfection of miR-34a into uveal melanoma cells led to a significant decrease in cell growth and migration. Following identification of two putative miR-34a binding sites within the 3' UTR of the human c-Met mRNA, miR-34a was shown to suppress luciferase activity using HEK293 cells with a luciferase reporter construct containing the binding sites. miR-34a was confirmed to downregulate the expression of c-Met protein by Western blotting and immunofluorescence. Furthermore, introduction of miR-34a also downregulated phosphorylated Akt and cell cycle-related proteins. CONCLUSIONS. Our results demonstrate that miR-34a acts as a tumor suppressor in uveal melanoma cell proliferation and migration through downregulation of c-Met. |
Reference:
MicroRNA-34a inhibits uveal melanoma cell proliferation and migration through downregulation of c-Met. | PMID:19029026
Yan D, Zhou X, Chen X, Hu DN, Dong XD, Wang J, Lu F, Tu L, Qu J.
Invest Ophthalmol Vis Sci. 2009 Apr;50(4):1559-65. Epub 2008 Nov 21. |
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