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Friday, Mar.29, 2024
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miRNA: |
hsa-miR-377 |
Disease: |
diabetic nephropathy |
Relationship type: |
Causal |
Detection method for miRNA expression: |
Northern blot, qRT-PCR etc |
Expression pattern of miRNA: |
up-regulated |
Validated targets of miRNA from the reference: |
unknown |
Validated targets of miRNA from TarBase: |
unknown : More... |
Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: Here, we exposed cultured human and mouse mesangial cells to high glucose and transforming growth factor-beta to simulate the diabetic milieu. In these conditions in vitro, as well as in mouse diabetic nephropathy models in vivo, microRNA-377 was consistently up-regulated relative to controls. Through a combination of computational and biological approaches, we identified relevant miR-377 target genes. Although fibronectin was induced by miR-377, it was not a direct target of miR-377. However, miR-377 led to reduced expressions of p21-activated kinase and superoxide dismutase, which enhanced fibronectin protein production. Thus, overexpression of miR-377 in diabetic nephropathy indirectly leads to increased fibronectin protein production; as such, miR-377 can have a critical role in the pathophysiology of this prevalent human disease. |
Reference:
MicroRNA-377 is up-regulated and can lead to increased fibronectin production in diabetic nephropathy. | PMID:18716028
Wang Q, Wang Y, Minto AW, Wang J, Shi Q, Li X, Quigg RJ.
FASEB J. 2008 Dec;22(12):4126-35. Epub 2008 Aug 20. |
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