Friday, Mar.29, 2024
miRNA:

hsa-miR-16

Disease:

hepatocellular carcinoma (HCC)

Relationship type:

Causal

Detection method for miRNA expression:

northern blot, qRT-PCR etc

Expression pattern of miRNA:

up-regulated

Validated targets of miRNA from the reference:

BCL2

Validated targets of miRNA from TarBase:

BCL2,CGI-38 : More...

Predicted targets: MIRANDA, TARGETSCAN, PICTAR-VERT

 

Description:

By miRNA microarray analysis, EGCG treatment was found to modify the expressions of some of the miRNAs in human hepatocellular carcinoma HepG2 cells, 13 were up-regulated and 48 were down-regulated. miR-16 is one of the miRNAs up-regulated by EGCG and one of its target genes is confirmed to be the anti-apoptotic protein Bcl-2. EGCG treatment induced apoptosis and down-regulated Bcl-2 in HepG2 cells. Transfection with anti-miR-16 inhibitor suppressed miR-16 expression and counteracted the EGCG effects on Bcl-2 down-regulation and also induction of apoptosis in cells. Results from the present study confirm the role of miR-16 in mediating the apoptotic effect of EGCG and also support the importance of miRNAs in the regulation of the biological activity of EGCG.

 

 

Reference:

Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells. | PMID:19269153
Tsang WP, Kwok TT.
J Nutr Biochem. 2009 Mar 5. [Epub ahead of print]

 

 
 
 
  miRBase
  Tarbase
  miRGen
  miRGator
  CCBB
  MicroRNA.org
  miRRim
  miRNAMap 2.0
 
 
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