Thursday, Mar.28, 2024
miRNA:

hsa-miR-106b

Disease:

hepatocellular carcinoma (HCC)

Relationship type:

Causal

Detection method for miRNA expression:

northern blot, qRT-PCR etc

Expression pattern of miRNA:

up-regulated

Validated targets of miRNA from the reference:

E2F1

Validated targets of miRNA from TarBase:

unknown : More...

Predicted targets: MIRANDA, TARGETSCAN, PICTAR-VERT

 

Description:

In this study, we examined and confirmed the over-expression of these clusters in hepatocellular carcinoma and in hepatoma-derived cells. At least 50% of the tumor samples showed a greater than two-fold increase in the expression for miR-18 and for the miR-106b-25 cluster when compared with the corresponding paired non-tumor samples. Knock-down studies for the miR-106b-25 cluster, which includes miR-106b, miR-93 and miR-25, showed that the expression of the cluster is necessary for cell proliferation and for anchorage-independent growth. In tumors with high expression of this cluster, reduced expression of the BH3-only protein Bim, a miR-25 target, was observed. We further identified the transcription factor E2F1 as a target gene for miR-106b and miR-93 and it is likely that one of the roles of the miR-106b-25 cluster is to prevent excessively high E2F1 expression, which may then cause apoptosis. We conclude that there is aberrant expression of microRNAs encoded by the oncogenic miR-17-92 cluster and the miR-106b-25 cluster in hepatocellular carcinoma. The consistent overexpression of the miR-106b-25 cluster and its role in cell proliferation and anchorage-independent growth points to the oncogenic potential of this cluster.

 

 

Reference:

Role of the miR-106b-25 microRNA cluster in hepatocellular carcinoma. | PMID:19486339
Li Y, Tan W, Neo TW, Aung MO, Wasser S, Lim SG, Tan TM.
Cancer Sci. 2009 Apr 15. [Epub ahead of print]

 

 
 
 
  miRBase
  Tarbase
  miRGen
  miRGator
  CCBB
  MicroRNA.org
  miRRim
  miRNAMap 2.0
 
 
Please don't hesitate to address comments/questions/suggestions regarding this webpage to: ydwang@hit.edu.cn or yunliu@iu.edu