|
|
|
|
Friday, Apr.26, 2024
|
|
|
|
|
|
| miRNA: |
hsa-miR-34a |
| Disease: |
retinoblastoma |
Relationship type: |
Causal |
Detection method for miRNA expression: |
northern blot, qRT-PCR etc |
| Expression pattern of miRNA: |
down-regulated |
Validated targets of miRNA from the reference: |
unknown |
Validated targets of miRNA from TarBase: |
E2F3 : More... |
| Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: Differential miR-34a and miR-34b expression was observed in retinoblastoma cell lines and tumor samples. miR-34a expression could be increased in Y79 cells, but not Weri-Rb1 cells, after p53 activation. This differential regulation was not due to genomic alterations at the miR-34a p53 binding site or mature gene. Exogeneous miR-34a inhibited Y79 and Weri-Rb1 cell growth and increased apoptotic activity in Y79 cells. Increased inhibition of Y79 and Weri-Rb1 cell growth was observed with combination miR-34a and topotecan treatment. We observed mRNA expression changes in 7 of 7 previously reported and 13 of 18 GenMiR++-predicted miR-34a targets after transfection of Y79 cells with miR-34a as compared to negative control microRNA. Conclusions: miR-34a functions as a tumor suppressor in retinoblastoma cells and is a potential therapeutic target. Differential expression, regulation, and activity of miR-34a in retinoblastoma cells may suggest further p53 pathway inactivation in retinoblastoma. |
Reference:
Differential MicroRNA-34a Expression and Tumor Suppressor Function in Retinoblastoma Cells. | PMID:19443717
Dalgard CL, Gonzalez M, Deniro JE, O'Brien JM.
Invest Ophthalmol Vis Sci. 2009 May 14. [Epub ahead of print] |
|
|
|
|
|
|
