hsa-miR-206

rhabdomyosarcoma

Relationship type:

Causal

Detection method for miRNA expression:

northern blot, qRT-PCR etc

down-regulated

Validated targets of miRNA from the reference:

c-Met

Validated targets of miRNA from TarBase:

Fstl1,GJA1,Utrn : More...

Description:

Due to the tissue specific nature of miR-1/206 for skeletal muscles, we investigated the role of miR-1/206 in the development of rhabdomyosarcoma. Initially, we demonstrated that miR-1/206 expression was suppressed in rhabdomyosarcomas and found at very low levels in a rhabdomyosarcoma RD cell line. Transient transfection of miR-1/206 into cultured RD cells led to a significant decrease in cell growth and migration. Using bioinformatics, we identified two putative miR-1/206 binding sites within the 3-untranslated region (3-UTR) of the human c-Met mRNA. miR-1/206 was then shown to have activity on mRNA expression by targeting the c-Met 3-UTR. The expression of c-Met protein was shown to be downregulated by subsequent Western blot analysis. Conversely, upregulation of c-Met was confirmed in tissue samples of human rhabdomyosarcoma, with its level inversely correlated with miR-1/206 expression. In vivo, miR-1/206 expressing tumor cells showed growth delay in comparison to negative control. Our results demonstrated that miR-1/206 suppressed c-Met expression in rhabdomyosarcoma and could function as a potent tumor suppressor in c-Met overexpressing tumors. Inhibition of miR-1/206 function could contribute to aberrant cell proliferation and migration, leading to rhadomyosarcoma development.

Reference:

MicroRNA-1/206 targets c-Met and inhibits rhabdomyosarcoma development. | PMID:19710019
Yan D, Dong XD, Chen X, Wang L, Lu C, Wang J, Qu J, Tu L.
J Biol Chem. 2009 Aug 26.