hsa-miR-107

non-small cell lung cancer (NSCLC)

Relationship type:

Causal

Detection method for miRNA expression:

northern blot, qRT-PCR etc

down-regulated

Validated targets of miRNA from the reference:

unknown

Validated targets of miRNA from TarBase:

unknown : More...

Description:

Introduction of synthetic miR-107 or miR-185 suppressed growth of the human non-small cell lung cancer cell lines. Flow cytometry analysis revealed these miRNAs induce a G1 cell cycle arrest in H1299 cells and the suppression of cell cycle progression is stronger than that by Let-7 miRNA. By the gene expression analyses with oligonucleotide microarrays, we find hundreds of genes are affected by transfection of these miRNAs. Using miRNA-target prediction analyses and the array data, we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6.

Reference:

MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines. | PMID:19688090
Takahashi Y, Forrest AR, Maeno E, Hashimoto T, Daub CO, Yasuda J.
PLoS One. 2009 Aug 18;4(8):e6677.