|
|
|
|
Thursday, Apr.25, 2024
|
|
|
|
|
|
| miRNA: |
hsa-miR-214 |
| Disease: |
cervical cancer |
Relationship type: |
Causal |
Detection method for miRNA expression: |
northern blot, qRT-PCR etc |
| Expression pattern of miRNA: |
down-regulated |
Validated targets of miRNA from the reference: |
MEK3, JNK1 |
Validated targets of miRNA from TarBase: |
unknown : More... |
| Predicted targets: |
MIRANDA, TARGETSCAN, PICTAR-VERT |
Description: We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3'UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs. |
Reference:
MicroRNA-214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells | PMID:19859982
Yang Z, Chen S, Luan X, Li Y, Liu M, Li X, Liu T, Tang H.
IUBMB Life. 2009 Oct 26;61(11):1075-1082. [Epub ahead of print] |
|
|
|
|
|
|
